Synaptojanin 1 antibody (Intracellular)

Catalog No. ABIN7885553

The Rabbit Polyclonal anti-Synaptojanin 1 antibody (ABIN7885553) specifically detects Synaptojanin 1 in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Synaptojanin 1 antibody (Intracellular) (ABIN7885553)

Target: Synaptojanin 1 (SYNJ1)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptojanin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Synaptojanin 1 Antibody

Binding Specificity: AA 1156-1171, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Synaptojanin 1

Predicted Reactivity: Rat - identical. Human - 15 out of 16 amino acids identical.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EAPKSPGTARKDNIGR, corresponding to amino acid residues 1156 - 1171 of mouse SYNJ1

Isotype: IgG

Application Details

Application Notes: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Synaptojanin 1

Target: Synaptojanin 1 (SYNJ1)

Alternative Name: SYNJ1

Background:

Synonyms: Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1, INPP5G, PARK20, SYNJ1

Description: Synaptojanin 1 (SYNJ1) is a phosphoinositide phosphatase primarily found in nerve terminals, where it plays a critical role in regulating synaptic vesicle recycling and neurotransmission, membrane trafficking and actin cytoskeleton dynamics. It belongs to the synaptojanin protein family, which includes other isoforms (SYNJ2 and SYNJ2BP) that also participate in cellular membrane dynamics and endocytic processes.1 Synaptojanin 1 is unique among phosphoinositide phosphatases in that it contains two catalytic domains that act on different substrates within the phosphoinositide signaling pathway. The SAC1 domain hydrolyzes phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 4-phosphate (PI4P), while the 5-phosphatase domain dephosphorylates phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂) to PI4P and phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P₃) to PI(3,4)P₂. 1,2 The coexistence of SAC1 and 5-phosphatase domains enables Synaptojanin 1 to orchestrate multiple steps in membrane trafficking across different subcellular compartments including vesicle uncoating, membrane trafficking, receptor recycling, and autophagy, processes fundamental to neuronal health, synaptic transmission, and plasticity.1,2,3 Based on its key function Synaptojanin 1 emerged as a key player in both short-term synaptic dynamics and long-term synaptic remodeling. In fact, Synaptojanin 1 dysfunction has been implicated in several neurodevelopmental and neurodegenerative disorders.3 Mutations in Synaptojanin 1 have been identified to result in comorbidities of early-onset Parkinsonism and epilepsy. The condition is now classified as PARK20, an autosomal recessive, juvenile-onset parkinsonism that presents with bradykinesia, dystonia, seizures, and cognitive impairment.3,4 Elevated levels of Synaptojanin 1, have been found in both human Alzheimer's disease (AD) brains, as well as mouse AD models and are correlated with cognitive decline linked to age.3,5

Gene ID: 104015

UniProt: Q8CHC4

Pathways: Inositol Metabolic Process, Synaptic Vesicle Exocytosis