STX2 antibody (N-Term)

Catalog No. ABIN7885558

The Rabbit Polyclonal anti-STX2 antibody is suitable to detect STX2 in samples from Mouse. It has been validated for WB, IHC and IF.

Quick Overview for STX2 antibody (N-Term) (ABIN7885558)

Target: STX2 (Syntaxin 2 (STX2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-STX2 Antibody

Binding Specificity: AA 2-19, N-Term

Purpose: A Rabbit Polyclonal Antibody to Syntaxin 2

Predicted Reactivity: Human - 16,17 amino acid residues identical, mouse - 15

Purification: Affinity purified on immobilized antigen.

Immunogen: RDRLPDLTACRKSDDGDN, corresponding to amino acid residues 2-19 of rat Syntaxin 2

Isotype: IgG

Application Details

Application Notes:

WB: 1:600

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237339)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for STX2

Target: STX2 (Syntaxin 2 (STX2))

Alternative Name: STX2

Background:

Synonyms: STX2, Epimorphin, EPIM

Description: Syntaxin 2 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 2, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 2 functions as a plasma membrane protein t-SNARE, which together with vesicular v-SNARE proteins is involved in regulated exocytosis in cells such as platelets, sperm cells and pancreatic acinar cells3-5. Intriguingly, Syntaxin 2 has been also identified as epimorphin, an extracellular membrane protein implicated in branching morphogenesis, a developmental process essential for the formation of many organs, including mammary gland, lung, kidney, and salivary gland6.

Gene ID: 25130

UniProt: P50279