STX3 antibody (Cytosolic, N-Term)

Catalog No. ABIN7885559

The Rabbit Polyclonal anti-STX3 antibody (ABIN7885559) specifically detects STX3 in WB, IF and IC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for STX3 antibody (Cytosolic, N-Term) (ABIN7885559)

Target: STX3 (Syntaxin 3 (STX3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Grade: Carrier-free, KO Validated

Product Details anti-STX3 Antibody

Binding Specificity: AA 2-16, Cytosolic, N-Term

Purpose: A Rabbit Polyclonal Antibody to Syntaxin 3

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: KDRLEQLKAKQLTQD(C), corresponding to amino acid residues 2-16 of rat syntaxin 3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237343)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for STX3

Target: STX3 (Syntaxin 3 (STX3))

Alternative Name: STX3

Background:

Synonyms: STX3

Description: Syntaxin 3 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2. SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 3, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 3 functions as a plasma membrane protein t-SNARE that is involved in epithelial cell polarization. Indeed, Syntaxin 3 is specifically localized in the apical membrane of polarized epithelial cells where it likely has a role in the polarized trafficking of newly synthesized proteins3.

Gene ID: 81802

UniProt: Q08849

Pathways: Synaptic Vesicle Exocytosis