Tetraspanin 2 antibody (Extracellular, Loop 2)

Catalog No. ABIN7885571

The Rabbit Polyclonal anti-Tetraspanin 2 antibody (ABIN7885571) specifically detects Tetraspanin 2 in WB, FACS, IHC and LCI. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Tetraspanin 2 antibody (Extracellular, Loop 2) (ABIN7885571)

Target: Tetraspanin 2 (TSPAN2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tetraspanin 2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Tetraspanin 2 Antibody

Binding Specificity: AA 114-130, Extracellular, Loop 2

Purpose: A Rabbit Polyclonal antibody to Tetraspanin 2 (extracellular)

Predicted Reactivity: Rat - identical,Human - 14 out of 17 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DVAIRHVQSMYEEAYSD, corresponding to amino acid residues 114 - 130 of mouse TSPAN2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Tetraspanin 2

Target: Tetraspanin 2 (TSPAN2)

Alternative Name: Tetraspan NET-3

Background:

Synonyms: Tetraspan NET-3, TSPAN2

Description: Tetraspanin 2 (TSPAN2) is a member of the Tetraspanin family of proteins, that comprises 33 members in humans. The family is characterized by the presence of four transmembrane domains, which give rise to the name "tetraspanin", as well as the presence two extracellular loops (EC1 and EC2), a small cytoplasmic loop, and short N- and C-terminal tails 1,2.Tetraspanins are able to interact with each other and also with numerous partner proteins such as integrins, growth factor receptors and ion channels, to create a dynamic network of interactions known as the tetraspanin-enriched microdomains (TEMs). Consequently, tetraspanins are regarded as molecular organizers and by forming complexes with these proteins, tetraspanins can modulate signal transduction pathways and cellular responses 2,3,4.Although TSPAN2 shares a relatively high homology with well-studied tetraspanins like CD9 (TSPAN29) or CD81 (TSPAN28), it has been less well-characterised up until now 3.Nevertheless, TSPAN2, like the related CD9 and CD81 proteins, has been described in relation to exosome formation, specifically regarding neuroinflammation and microglia activation 5, as well as oligodendrocyte differentiation into myelin-forming glia and in the progression of tumor metastasis by modulating cancer cell motility and invasion 3,5.

Gene ID: 70747

UniProt: Q922J6