TMEM119 antibody (Extracellular, N-Term)

Catalog No. ABIN7885617

This Rabbit Polyclonal antibody specifically detects TMEM119 in FACS and WB. It exhibits reactivity toward Mouse and Rat.

Quick Overview for TMEM119 antibody (Extracellular, N-Term) (ABIN7885617)

Target: TMEM119 (Transmembrane Protein 119 (TMEM119))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMEM119 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB)

Grade: Carrier-free

Product Details anti-TMEM119 Antibody

Binding Specificity: AA 53-67, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to TMEM119 (extracellular)

Predicted Reactivity: Rat - 12,15 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237517)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for TMEM119

Target: TMEM119 (Transmembrane Protein 119 (TMEM119))

Alternative Name: Transmembrane Protein 119

Background:

Synonyms: Transmembrane Protein 119, Osteoblast induction factor, OBIF

Description: Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

Gene ID: 231633

UniProt: Q8R138