TMEM66 antibody (Intracellular, Lumenal Region)

Catalog No. ABIN7885623

This Rabbit Polyclonal antibody specifically detects TMEM66 in WB, IF and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TMEM66 antibody (Intracellular, Lumenal Region) (ABIN7885623)

Target: TMEM66 (Transmembrane Protein 66 (TMEM66))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMEM66 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-TMEM66 Antibody

Binding Specificity: AA 141-155, Intracellular, Lumenal Region

Purpose: A Rabbit Polyclonal Antibody to TMEM66

Predicted Reactivity: 15 amino acid residues identical,Mouse - 13, human - 11

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ELGLSKLKESGKHQS, corresponding to amino acid residues 141-155 of rat SARAF

Isotype: IgG

Application Details

Application Notes:

WB: 1:500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237528)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for TMEM66

Target: TMEM66 (Transmembrane Protein 66 (TMEM66))

Alternative Name: Store-operated calcium entry-associated regulatory factor

Background:

Synonyms: Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66

Description: TMEM66 (Transmembrane protein 66, also known as SOCE-associated regulatory factor, SARAF) is a regulatory protein of cellular Ca2+ homeostasis, encoded by the TMEM66 gene. TMEM66 is an ER resident protein, which responds to cytosolic Ca2+ elevation after ER Ca2+ refilling.TMEM66 is highly conserved in vertebrates. In mammals it is ubiquitously expressed with high transcript levels in the immune and neuronal tissues. The TMEM66 structure contains validated signal peptide and a putative single membrane spanning domain, a serine-proline rich domain and arginine rich regions followed by a cluster of basic residues at its C-terminal tail1.Store-operated Ca2+ entry (SOCE) is an important Ca2+ influx pathway across plasma membranes in many non-excitable cells. In this pathway, intracellular Ca2+ release through IP3 receptors stimulated by a variety of agonists, results in reduction of Ca2+ concentration in the lumen of the endoplasmic or sarcoplasmic reticulum. SARAF negatively regulates store operated calcium entry into cells and protects cells from calcium overfilling1,2.STIM, Ca2+ sensor, and Orai, the channel pore forming subunit proteins have been identified as the essential components enabling the reconstitution of Ca2+ release-activated Ca2+ channels that mediate SOCE. Knock-out of Orai1 or STIM1 in mice and mutations evoke SOCE in lymphocytes and other cells2,3.Findings show that dysregulation of Ca2+ homeostasis is associated with a plethora of pathological conditions including neurodegenerative diseases, skeletal, muscular and cardiovascular disorders2. Inherited defects in SOCE due to mutations in genes of the Ca2+ release-activated Ca2+ (CRAC) channel complex causes patients to suffer from a severe form of immunodeficiency that is due to defects in the function of T cells, NK cells and potentially other immune cells3.

Gene ID: 290796

UniProt: Q6AYN2