RCC1 antibody (pSer11) (Biotin)

Catalog No. ABIN745165

This Rabbit Polyclonal antibody specifically detects RCC1 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for RCC1 antibody (pSer11) (Biotin) (ABIN745165)

Target: RCC1 (Regulator of Chromosome Condensation 1 (RCC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RCC1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RCC1 Antibody (Biotin)

Binding Specificity: pSer11

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human RCC1 around the phosphorylation site of Ser11

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for RCC1

Target: RCC1 (Regulator of Chromosome Condensation 1 (RCC1))

Alternative Name: RCC1

Background:

Synonyms: Cell cycle regulatory protein, CHC 1, CHC1, Chromosome condensation 1, Chromosome condensation protein 1, Guanine nucleotide releasing protein, HERC2, Ran GEF, RanGEF, RCC 1, RCC1 I, Regulator of chromosome condensation 1, Regulator of chromosome condensation, SHEP1, RCC1_HUMAN.

Background: Ran GTPase plays important roles in nucleocytoplasmic transport in interphase and in both spindle formation and nuclear envelope (NE) assembly during mitosis. The latter functions rely on the presence of high local concentrations of GTP bound Ran near mitotic chromatin. RanGTP localization has been proposed to result from the association of Ran's GDP/GTP exchange factor, RCC1, with chromatin , but Ran is shown here to bind directly to chromatin in two modes, either dependent or independent of RCC1, and, where bound, to increase the affinity of chromatin for NE membranes.

Gene ID: 1104

Pathways: Chromatin Binding, Protein targeting to Nucleus