BRD9 antibody (C-Term)

Catalog No. ABIN7938071

The Rabbit Polyclonal anti-BRD9 antibody is suitable to detect BRD9 in samples from Human. It has been validated for WB and IP.

Quick Overview for BRD9 antibody (C-Term) (ABIN7938071)

Target: BRD9 (Bromodomain Containing 9 (BRD9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD9 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-BRD9 Antibody

Binding Specificity: C-Term

Purpose: BRD9 antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the C-terminal region of human BRD9 (bromodomain containing 9).

Isotype: IgG

Application Details

Application Notes: IP: 5 µL per IP WB*: 1:250 - 1:1,000 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal IP results, the addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for BRD9

Target: BRD9 (Bromodomain Containing 9 (BRD9))

Alternative Name: BRD9

Background:

Background_Plain: BRD9 (bromodomain containing 9) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6) that function in the regulation of transcriptional activation and chromatin remodeling. There are five isoforms of BRD9 that are produced by alternative splicing.

Aliases: PRO9856, LAVS3040

Molecular Weight: 85 kDa

NCBI Accession: NP_076413