GLYR1 antibody (C-Term)

Catalog No. ABIN7938142

The Rabbit Polyclonal anti-GLYR1 antibody (ABIN7938142) specifically detects GLYR1 in WB. The antibody is reactive with Human samples.

Quick Overview for GLYR1 antibody (C-Term) (ABIN7938142)

Target: GLYR1 (Glyoxylate Reductase 1 Homolog (GLYR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLYR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLYR1 Antibody

Binding Specificity: C-Term

Purpose: GLYR1 antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the C-terminal region of human GLYR1.

Isotype: IgG

Application Details

Application Notes: WB*: 0.5 - 2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris, 105 mM NaCl, 0.07 mM EDTA, 31 mM glycine, 30 % glycerol, and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for GLYR1

Target: GLYR1 (Glyoxylate Reductase 1 Homolog (GLYR1))

Alternative Name: GLYR1

Background:

Background_Plain: GLYR1 / NDF (nucleosome-destabilizing factor) that is recruited to gene bodies during transcriptional activation and facilitates Pol II transcription through nucleosomes, where it is proposed to ""unzip"" chromatin during transcription, pushing the polymerase past pause points. It was discovered as a factor that allows p300 to acetylate Histone H3K56, which is normally an inaccessible residue for this modification. GLYR1 is shown to associate with a subset of highly transcribed genes tending to have longer mRNAs rather than shorter, and is also at a significant subset of loci containing the Histone H3K36me3. GLYR1 is expressed at high levels in most tissue types, including stem cells, and has been found to be overexpressed in breast cancer.

Aliases: Glyoxylate Reductase 1 Homolog, HIBDL, HNDF, NDF

Molecular Weight: 65 kDa

NCBI Accession: NP_115958