Recombinant HDAC3 antibody

Catalog No. ABIN7938149

The Rabbit Monoclonal anti-HDAC3 antibody is suitable to detect HDAC3 in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for Recombinant HDAC3 antibody (ABIN7938149)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 2N1

Product Details anti-HDAC3 Antibody

Purpose: HDAC3 antibody (rAb), 100 μg (Clone 2N1)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against the HDAC3 fusion protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:50000 dilution IHC: 1:50-1:500 dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, pH 7.3, with 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background:

Background_Plain: Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (amino groups of lysine's located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene.

Aliases: HD3, RPD3, RPD3-2

Molecular Weight: 49 kDa

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development