HDAC3 antibody (pSer424)

Catalog No. ABIN7938153

The Rabbit Polyclonal anti-HDAC3 antibody (ABIN7938153) specifically detects HDAC3 in WB and DB. The antibody is reactive with Human and Mouse samples.

Quick Overview for HDAC3 antibody (pSer424) (ABIN7938153)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Product Details anti-HDAC3 Antibody

Binding Specificity: pSer424

Purpose: HDAC3 Phospho Ser424 antibody (pAb), sample

Purification: Protein A Chromatography

Immunogen: This HDAC3 antibody was raised against a synthetic peptide sequence phosphorylated at serine 424.

Isotype: IgG

Application Details

Application Notes: WB: 0.5 - 2 μg/mL DB: 0.5 - 2 μg/mL For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. For HDAC3, we also offer AbFlex HDAC3 Recombinant Antibody (rAb). For details, see Catalog No. 91199.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background:

Background_Plain: HDAC3 (Histone Deacetylase 3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). By forming multi-protein complexes with the co-repressors SMRT and N-CoR, HDAC3 regulates the transcription of a plethora of genes. A growing list of non-histone proteins extends the role of HDAC3 beyond transcriptional repression. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

Aliases: Phospho-HDAC3 (Ser424), Phospo-HDAC3

Molecular Weight: 50 kDa

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development