Histone H1 antibody (AA 109-256)

Catalog No. ABIN7938159

The Mouse Monoclonal anti-Histone H1 antibody (Clone 1G6-E3) (ABIN7938159) specifically detects Histone H1 in WB, IF, IP, ChIP and ChIP-seq. The antibody is reactive with Drosophila melanogaster samples.

Quick Overview for Histone H1 antibody (AA 109-256) (ABIN7938159)

Target: Histone H1

Reactivity: Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Clone: 1G6-E3

Product Details anti-Histone H1 Antibody

Binding Specificity: AA 109-256

Purpose: Histone H1 antibody (mAb) (Clone 1G6-E3), sample

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a recombinant protein containing amino acids 109-256 of Drosophila melanogaster Histone H1. This region of the protein is not conserved in mammals, the antibody is not likely to cross-react to human, mouse or rat Histone H1.

Isotype: IgG1

Application Details

Application Notes: WB: 0.2 - 1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone H1

Target: Histone H1

Background:

Background_Plain: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation and play a major role in regulating gene expression.

Aliases: 1G6-E3

Molecular Weight: 35 kDa

NCBI Accession: NP_001027286