Histone 3 antibody (H3K27me3)

Catalog No. ABIN7938230

The Rabbit Polyclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human, Mouse and Monkey. It has been validated for WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag and CUT&RUN.

Quick Overview for Histone 3 antibody (H3K27me3) (ABIN7938230)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K27me3

Purpose: Histone H3K27me3 antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a peptide including trimethyl-lysine 27 of histone H3.

Isotype: IgG

Application Details

Application Notes: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 μg each ICC/IF: 2 μg/mL dilution IHC(FFPE): 2 μg/mL dilution WB*: 0.5 - 2 μg/mL dilution CUT&Tag: 1 μg per 50 µL reaction* CUT&RUN: 1 μg per 50 µL reaction* *This antibody has been validated for CUT&Tag using Active Motif's CUT&Tag-IT Assay Kit, Catalog No. 53160. *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for WB. For H3K27me3, we also offer AbFlex H3K27me3 Recombinant Antibody (rAb). For details, see Catalog No. 91167.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

Molecular Weight: 17 kDa

NCBI Accession: NP_003522