Histone 3 antibody (H3K36me3)

Catalog No. ABIN7938238

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, ChIP, DB, ChIP-seq and CUT&Tag. It exhibits reactivity toward Human and Mouse.

Quick Overview for Histone 3 antibody (H3K36me3) (ABIN7938238)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K36me3

Purpose: Histone H3K36me3 antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This Histone H3 trimethyl Lys36 antibody was raised against a peptide containing trimethyl Lys36 of human histone H3.

Isotype: IgG

Application Details

Application Notes: ChIP: 10 μg per ChIP ChIP-Seq: 4 μg each WB: 0.5 - 2 μg/mL dilution CUT&Tag: 1 μg per 50 µL reaction For Histone H3K36me3, we also offer AbFlex Histone H3K36me3 Recombinant Antibody (rAb). For details, see Catalog No. 91265.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.

Molecular Weight: 17 kDa

NCBI Accession: NP_003522