Histone H4 antibody (H4K16ac)

Catalog No. ABIN7938255

The Rabbit Polyclonal anti-Histone H4 antibody (ABIN7938255) specifically detects Histone H4 in WB, IF, ChIP, DB, ChIP-seq and CUT&Tag. The antibody is reactive with Human, Mouse, Saccharomyces cerevisiae and Drosophila melanogaster samples.

Quick Overview for Histone H4 antibody (H4K16ac) (ABIN7938255)

Target: Histone H4 (HISTH4)

Reactivity: Human, Mouse, Saccharomyces cerevisiae, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Product Details anti-Histone H4 Antibody

Binding Specificity: H4K16ac

Purpose: Histone H4K16ac antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H4 acetyl Lys16 antibody was raised against a peptide including acetyl-lysine 16 of histone H4.

Application Details

Application Notes: ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB: 1:1,000 - 1:5,000 dilution CUT&Tag: 1-2 µL per 50 µL reaction ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. For optimal results in Western blotting, primary antibody incubations should be performed overnight at 4 °C. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. NoRC is a SMARCA5 (SNF2h)-containing chromatin remodeling complex. The bromodomain of TIP5, the large subunit of NoRC, interacts with acetylated Histone H4 Lys16, (H4K16ac) and cooperates with an adjacent PHD finger to recruit histone deacetylases and DNA methyltransferases to rDNA, leading to the silencing of rDNA.

Molecular Weight: 8 kDa

NCBI Accession: NP_778224