HNRNPC antibody

Catalog No. ABIN7938264

The Rabbit Polyclonal anti-HNRNPC antibody is suitable to detect HNRNPC in samples from Human. It has been validated for WB.

Quick Overview for HNRNPC antibody (ABIN7938264)

Target: HNRNPC (Heterogeneous Nuclear Ribonucleoprotein C (C1/C2) (HNRNPC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPC antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HNRNPC Antibody

Purpose: HNRNPC antibody (pAb)

Purification: Protein A Chromatography

Immunogen: Peptide containing a sequence fragment corresponding to human heterogeneous nuclear ribonucleoprotein C.

Isotype: IgG

Application Details

Application Notes: WB: 0.5 - 2 μg/mL For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for HNRNPC

Target: HNRNPC (Heterogeneous Nuclear Ribonucleoprotein C (C1/C2) (HNRNPC))

Alternative Name: HNRNPC

Background:

Background_Plain: HNRNPC (Heterogeneous Nuclear Ribonucleoprotein C) is a member of the subfamily nuclear ribonucleoproteins (hnRNPs) involved in pre-mRNA processing, mRNA metabolism, and transport. These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene (C1 and C2). HNRNPC is a critical alternative splicing regulator that complexes with heterogeneous nuclear RNA (hnRNA) and plays an essential role in RNA maturation, stabilization, translocation, and posttranscriptional modification. Cellular homeostasis, among other cellular processes, is regulated by RNA alternative splicing. Alternative splicing disfunction contributes to many disorders, including cancer. The up regulation of HNRNPC expression has been tied to cancer progression and is thought to function by regulating RNA splicing, RNA expression, RNA stability, mRNA translation, m6A RNA methylation, and dsRNA induced interferon response.

Aliases: Heterogeneous Nuclear Ribonucleoprotein C, hnRNP C, HNRPC

Molecular Weight: 37 kDa

UniProt: P07910