ING5 antibody

Catalog No. ABIN7938284

The Rat Monoclonal anti-ING5 antibody is suitable to detect ING5 in samples from Human. It has been validated for WB and IP.

Quick Overview for ING5 antibody (ABIN7938284)

Target: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This ING5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 7A11

Product Details anti-ING5 Antibody

Purpose: ING5 antibody (mAb) (Clone 7A11)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against full length recombinant human ING5 (Inhibitor of growth protein 5) protein.

Isotype: IgG2a

Application Details

Application Notes: WB: 0.5 - 2 μg/mL IP: 5 μg/mL This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91329.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for ING5

Target: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Alternative Name: ING5

Background:

Background_Plain: ING5 (Inhibitor of Growth 5) possesses different structural and functional elements, including a PHD (plant homeodomain), an NCR (novel conserved region), a LZL (leucine zipper like) finger, and two NLS (nuclear localization sequence) motifs. ING proteins bind to methylated core histones through their PHD motif, notably ING5 reads histone H3 when di- or trimethylated at lysine 4 (H3K4me2/3). ING5 is a subunit of two distinct histone acetyltransferase (HAT) complexes with HBO1 or MOZ/MORF as the two catalytic subunits. These complexes contain two different alternative scaffolding proteins, i.e. a BRPF protein or a JADE protein, that determine their specificity towards different core histones. ING complexes appear to participate in transcription, for example by interacting with transcriptional regulators such as the tumor suppressor p53 and through the ability of ING5 to recognize H3K4me3, a mark associated with open chromatin and transcribed genes.

Aliases: Inhibitor of growth protein 5, p28ING5

Molecular Weight: 30 kDa

NCBI Accession: NP_001317091

Pathways: Chromatin Binding