WDR4 antibody

Catalog No. ABIN7938332

The Rabbit Polyclonal anti-WDR4 antibody is suitable to detect WDR4 in samples from Human. It has been validated for WB.

Quick Overview for WDR4 antibody (ABIN7938332)

Target: WDR4 (WD Repeat Domain 4 (WDR4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WDR4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WDR4 Antibody

Purpose: WDR4 antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a human peptide containing WD repeat domain 4.

Isotype: IgG

Application Details

Application Notes: WB: 0.5 - 2 μg/mL *Note: For optimal results, we recommend the addition of 0.05 % Tween-20 to all blocking solutions to reduce background in Western blot.

Restrictions: For Research Use only

Handling

Buffer: Purified in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for WDR4

Target: WDR4 (WD Repeat Domain 4 (WDR4))

Alternative Name: WDR4

Background: WDR4 (WD Repeat-Containing Protein 4), also known as TRM82, is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. WDR4 is the non-catalytic subunit of N7-methylguanosine (m7G). WRD4 and METTL1 form a complex required for tRNA (guanine(46)-N(7))-methyltransferase activity. This complex catalyzes tRNA m7G methylation to enhance translation efficiency.

Molecular Weight: 45 kDa