METTL3 antibody (N-Term)

Catalog No. ABIN7938336

The Mouse Monoclonal anti-METTL3 antibody is suitable to detect METTL3 in samples from Human. It has been validated for WB and IP.

Quick Overview for METTL3 antibody (N-Term) (ABIN7938336)

Target: METTL3 (Methyltransferase Like 3 (METTL3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This METTL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 29C8

Product Details anti-METTL3 Antibody

Binding Specificity: N-Term

Purpose: METTL3 antibody (mAb) (Clone 29C8)

Purification: Unpurified

Immunogen: This antibody was raised against a peptide within the N-terminal region of human METTL3 (N6-adenosine-methyltransferase).

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500 - 1:1000 dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91333.

Restrictions: For Research Use only

Handling

Buffer: This antibody is provided as a hybridoma culture supernatant with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for METTL3

Target: METTL3 (Methyltransferase Like 3 (METTL3))

Alternative Name: METTL3

Background:

Background_Plain: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also occurs in other RNA molecules, such as primary miRNA (pri-miRNAs). METTL3 (methyltransferase-like 3, also known as IME4, M6A, MT-A70) forms a stable N6-methyltransferase heterodimer complex with METTL14, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition. METTL3 promotes translation by interacting with the translation initiation machinery in the cytoplasm. Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation.

Aliases: Methyltransferase Like 3, MRNA (2-O-Methyladenosine-N(6)-)-Methyltransferase

Molecular Weight: 72 kDa

NCBI Accession: NP_062826