METTL16 antibody

Catalog No. ABIN7938345

The Rabbit Polyclonal anti-METTL16 antibody is suitable to detect METTL16 in samples from Human. It has been validated for WB.

Quick Overview for METTL16 antibody (ABIN7938345)

Target: METTL16 (Methyltransferase Like 16 (METTL16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METTL16 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-METTL16 Antibody

Purpose: METTL16 antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This METTL16 antibody was raised against a fusion protein corresponding to human METTL16.

Isotype: IgG

Application Details

Application Notes: WB: 0.5 μg/mL-2 μg/mL For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for METTL16

Target: METTL16 (Methyltransferase Like 16 (METTL16))

Alternative Name: METTL16

Background:

Background_Plain: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also occurs in other RNA molecules, such as primary miRNA (pri-miRNAs). METTL16 (Methyltransferase-Like Protein 16) is an RNA N6-methyltransferase that methylates adenosine residues of a subset of mRNAs and U6 smaller nuclear RNAs. METTL16 controls a significant portion of m6A epitranscriptome by regulating SAM homeostasis and plays a role in S-adenosyl-L-methionine homeostasis by regulating the expression of MAT2A transcripts.

Aliases: Methyltransferase-Like Protein 16

Molecular Weight: 64.9 kDa

NCBI Accession: NP_076991