NAT10 antibody

Catalog No. ABIN7938364

This Rabbit Polyclonal antibody specifically detects NAT10 in WB. It exhibits reactivity toward Human.

Quick Overview for NAT10 antibody (ABIN7938364)

Target: NAT10 (N-Acetyltransferase 10 (GCN5-Related) (NAT10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAT10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NAT10 Antibody

Purpose: NAT10 antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a human RNA cytidine acetyltransferase isoform a.

Isotype: IgG

Application Details

Application Notes: WB: 1 - 4 μg dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG supplied in 62 mM Trisodium citrate, 87 mM Tris pH 7, 30 % glycerol, 0.035 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for NAT10

Target: NAT10 (N-Acetyltransferase 10 (GCN5-Related) (NAT10))

Alternative Name: NAT10

Background:

Background_Plain: NAT10 (N-Acetyltransferase 10) antibody is a RNA cytidine acetyltransferase that catalyzes the formation of N(4)- acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs and mediates the formation of ac4C at position 1842 in 18S rRNA. NAT10 requires the tRNA-binding adapter protein THUMPD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. In addition to RNA acetyltransferase activity, NAT10 is also able to acetylate lysine residues of proteins, such as histones, microtubules, p53/TP53 and MDM2, in vitro. It activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. NAT10 involved in the regulation of centrosome duplication by acetylating CENATAC during mitosis, promoting SASS6 proteasome degradation.

Aliases: N-Acetyltransferase 10

Molecular Weight: 130 kDa

NCBI Accession: NP_078938