Recombinant NAT10 antibody

Catalog No. ABIN7938367

This Rabbit Monoclonal antibody specifically detects NAT10 in WB. It exhibits reactivity toward Human and Rat.

Quick Overview for Recombinant NAT10 antibody (ABIN7938367)

Target: NAT10 (N-Acetyltransferase 10 (GCN5-Related) (NAT10))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NAT10 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 5B9

Product Details anti-NAT10 Antibody

Purpose: NAT10 antibody (rAb), 100 μg (Clone 5B9)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against the NAT10 fusion protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:50000 dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, pH 7.3, with 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for NAT10

Target: NAT10 (N-Acetyltransferase 10 (GCN5-Related) (NAT10))

Alternative Name: NAT10

Background:

Background_Plain: NAT10 (N-Acetyltransferase 10) is a RNA cytidine acetyltransferase that catalyzes the formation of N(4)- acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs and mediates the formation of ac4C at position 1842 in 18S rRNA. NAT10 requires the tRNA-binding adapter protein THUMPD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. In addition to RNA acetyltransferase activity, ,strong>NAT10 is also able to acetylate lysine residues of proteins, such as histones, microtubules, p53/TP53 and MDM2, in vitro. It activates telomerase activity by stimulating the transcription of TERT and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. NAT10 involved in the regulation of centrosome duplication by acetylating CENATAC during mitosis, promoting SASS6 proteasome degradation.

Aliases: N-Acetyltransferase 10

Molecular Weight: 116 kDa