PCIF1 antibody (C-Term)

Catalog No. ABIN7938398

The Rabbit Polyclonal anti-PCIF1 antibody is suitable to detect PCIF1 in samples from Human. It has been validated for WB and IP.

Quick Overview for PCIF1 antibody (C-Term) (ABIN7938398)

Target: PCIF1 (PDX1 C-terminal Inhibiting Factor 1 (PCIF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCIF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-PCIF1 Antibody

Binding Specificity: C-Term

Purpose: PCIF1/CAPAM antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a peptide within the C-terminal region of human PCIF1.

Isotype: IgG

Application Details

Application Notes: IP: 10 µL per IP WB*: 0.5-2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal IP results, the addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, 30 % glycerol, and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for PCIF1

Target: PCIF1 (PDX1 C-terminal Inhibiting Factor 1 (PCIF1))

Alternative Name: PCIF1

Background:

Background_Plain: The abundant modification N6-methyladenosine (m6A), in eukarytic mRNAs, has been identified as a major epitranscriptome mark that plays critical roles in RNA metabolism and function. In addition to the internal m6A, N6, 2-O dimethyladenosine (m6Am) is present at the transcription start site of capped mRNAs in vertebrates. The abundance of this modification in the transcriptome has been quantified and identified as the writer protein, cap-specific adenosine methyltransferase (CAPAM/PCIF1), is needed for this modification. CAPAM/PCIF1 is identified as the Methyltransferase responsible for methylating 2-O-methyladenosine (Am) at its N6 position to generate m6Am. m6Am is involved in regulating translation of capped mRNAs. Depleting CAPAM/PCIF1 does not affect mRNA translation but is associated with reduced stability of a subset of m6Am annotated mRNAs.

Aliases: Transcription factor PCF1, Phosphorylated CTD Interacting Factor 1 (PCIF1)

Molecular Weight: 80 kDa

NCBI Accession: NP_071387