POLR2F antibody

Catalog No. ABIN7938437

The Mouse Monoclonal anti-POLR2F antibody is suitable to detect POLR2F in samples from Human, Rat, Mouse, C. elegans and Saccharomyces cerevisiae. It has been validated for WB, IF, ChIP, ChIP-seq and ICC.

Quick Overview for POLR2F antibody (ABIN7938437)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivity: Human, Rat, Mouse, C. elegans, Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunocytochemistry (ICC)

Clone: 4H8

Product Details anti-POLR2F Antibody

Purpose: RNA pol II antibody (mAb) (Clone 4H8)

Purification: Affinity Purified

Immunogen: This RNA pol II antibody was raised against a synthetic peptide YSPTSpPS corresponding to human RNA pol II. It recognizes both the phosphorylated and unphosphorylated forms of the largest subunit of RNA pol II.

Isotype: IgG1

Application Details

Application Notes: ChIP: 10 µL per ChIP ChIP-Seq: 6-20 µL each WB: 1:2,000 - 1:5,000 dilution For optimal results in Western blotting, primary antibody incubations should be performed overnight at 4 °C. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.2 % gelatin, 0.01 % thimerosal, 0.1 % sodium azide, and 50 % glycerol. Thimerosal and sodium azide are highly toxic.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: RNA pol II

Background:

Background_Plain: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220

Molecular Weight: 217 kDa

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways