POLR2F antibody (pThr4)

Catalog No. ABIN7938442

This Rat Monoclonal antibody specifically detects POLR2F in WB, IF, ELISA, ChIP, IP, ICC and DB. It exhibits reactivity toward Human.

Quick Overview for POLR2F antibody (pThr4) (ABIN7938442)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 6D7

Product Details anti-POLR2F Antibody

Binding Specificity: pThr4

Purpose: RNA pol II CTD phospho Thr4 antibody (mAb) (Clone 6D7)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide corresponding to RNA Pol II CTD phosphorylated at threonine 4.

Isotype: IgG2b

Application Details

Application Notes: WB: 0.5 - 2 μg/mL DB: 1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: RNA pol II

Background:

Background_Plain: RNA pol II (RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair. RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation.

Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220

Molecular Weight: 240 kDa

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways