POLR2F antibody (meLys7)

Catalog No. ABIN7938446

This Rat Monoclonal antibody specifically detects POLR2F in WB, ChIP, IP and ChIP-seq. It exhibits reactivity toward Human.

Quick Overview for POLR2F antibody (meLys7) (ABIN7938446)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Clone: 1F5

Product Details anti-POLR2F Antibody

Binding Specificity: meLys7

Purpose: RNA pol II CTD monomethyl Lys7 antibody (mAb) (Clone 1F5)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a peptide containing dimethyl-lysine 7 of human RNA Pol II CTD.

Isotype: IgG2a

Application Details

Application Notes: ChIP-Seq: 10 μg per ChIP IP: 5 μg per IP WB*: 0.5 - 2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.05 % BSA and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: RNA pol II

Background:

Background_Plain: Genome-wide profiling revealed high levels of K7me1/2 marks at the transcriptional start site of genes for sense and antisense transcribing RNAPII. The new K7 modifications further expand the mammalian CTD code to allow regulation of differential gene expression. In analogy, it appears likely that the various modifications of K7 residues identified in this analysis may similarly contribute to fine tuning of gene expressing by RNAPII, and that these modifications are placed in K7 residues of CTD dependent on cell signaling and epigenetic programming of cells.

Aliases: RNA Polymerase II Carboxy Terminal Domain

Molecular Weight: 240 kDa

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways