POLR2F antibody (Cit1810)

Catalog No. ABIN7938450

This Rabbit Polyclonal antibody specifically detects POLR2F in WB, IP and DB. It exhibits reactivity toward Human.

Quick Overview for POLR2F antibody (Cit1810) (ABIN7938450)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Product Details anti-POLR2F Antibody

Binding Specificity: Cit1810

Purpose: RNA pol II CTD Cit1810 antibody (pAb)

Purification: Affinity Purified

Immunogen: This RNA pol II CTD Cit 1810 antibody was raised against a peptide containing the RNA Pol II CTD sequence containing citrulline and corresponding to human RNA pol ll.

Isotype: IgG

Application Details

Application Notes: IP: 4 - 10 µL per IP WB: 1:500 - 1:2,000 dilution DB: 1:500 dilution For RNA pol II CTD , we also offer AbFlex RNA pol II CTD Recombinant Antibody (rAb). For details, see Catalog No. 91151.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris, 32 mM NaOAC, 0.035 % sodium azide, 30 % glycerol and 0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: RNA pol II

Background:

Background_Plain: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. This antibody is specific for the deamination of arginine and its conversation to citrulline by peptidyl arginine deiminase 2 (PADI2), an enzyme that has been associated with several diseases, including cancer. Among the PADI family members, only PADI2 citrullinates R1810 (Cit1810) at repeat 31 of the CTD. Loss of R1810 result in increased amounts of RNAP2 at transcription start cites, reduce gene expression, and inhibits cell proliferation.

Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220

Molecular Weight: 210 kDa

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways