SMC3 antibody (acLys105, acLys106, C-Term)

Catalog No. ABIN7938499

This Mouse Monoclonal antibody specifically detects SMC3 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for SMC3 antibody (acLys105, acLys106, C-Term) (ABIN7938499)

Target: SMC3 (Structural Maintenance of Chromosomes 3 (SMC3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMC3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMC3 Antibody

Binding Specificity: acLys105, acLys106, C-Term

Purpose: SMC3 K105acK106ac antibody (mAb)

Purification: Protein G Chromatography

Immunogen: This SMC3 antibody was raised against a peptide in the C-terminal region of human SMC3.

Isotype: IgG1

Application Details

Application Notes: WB*: 1:500 - 1:1,000 dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, 30 % glycerol, 0.035 % Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for SMC3

Target: SMC3 (Structural Maintenance of Chromosomes 3 (SMC3))

Alternative Name: SMC3

Background:

Background_Plain: The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression.SMC3 is a component of the cohesin complex that plays an essential role during chromatid segregation. SMC3 is also involved in DNA recombination and repair, mitotic chromosome organization and microtubule-mediated intracellular transport. SMC3 is acetylated during replication, which is essential for cohesion, and is phosphorylated by ATM after DNA double strand breakage. This antibody is acetylated at lysine 105/106.SMC3 is frequently elevated in human colon carcinoma and overexpression of the protein transforms fibroblasts.

Aliases: BAM, BMH, HCAP, CDLS3, CSPG6, SMC3L1

Molecular Weight: 141 kDa

NCBI Accession: NP_005436

Pathways: Stem Cell Maintenance