SUPT5H antibody (C-Term)

Catalog No. ABIN7938513

The Rat Monoclonal anti-SUPT5H antibody is suitable to detect SUPT5H in samples from Human. It has been validated for WB, IP and ChIP-seq.

Quick Overview for SUPT5H antibody (C-Term) (ABIN7938513)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This SUPT5H antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Clone: 6F1

Product Details anti-SUPT5H Antibody

Binding Specificity: C-Term

Purpose: Supt5H/SPT5 antibody (mAb) (Clone 6F1)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a synthetic peptide derived from the human SPT5 C-terminal region.

Isotype: IgG1

Application Details

Application Notes: ChIP-Seq: 4 μg per ChIP WB*: 0.5 - 2 μg/mL IP: 5 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91331.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for SUPT5H

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Alternative Name: Supt5H/SPT5

Background:

Background_Plain: SUPT5H (SPT5 Homolog, DSIF Elongation Factor Subunit) is a transcription elongation factor which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. SUPT5H also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Diseases associated with SUPT5H include HIV-1. Among its related pathways are mRNA capping and HIV life cycle.

Aliases: SPT5 Homolog, DSIF Elongation Factor Subunit, DSIF Large Subunit, Transcription Elongation Factor SPT5

Molecular Weight: 140 kDa

NCBI Accession: NP_001104490