SUZ12 antibody (C-Term)

Catalog No. ABIN7938516

The Rabbit Polyclonal anti-SUZ12 antibody (ABIN7938516) specifically detects SUZ12 in WB, IF, ChIP, ChIP-seq and CUT&RUN. The antibody is reactive with Human and Mouse samples.

Quick Overview for SUZ12 antibody (C-Term) (ABIN7938516)

Target: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUZ12 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Product Details anti-SUZ12 Antibody

Binding Specificity: C-Term

Purpose: Suz12 antibody (pAb)

Purification: Affinity Purified

Immunogen: This Suz12 antibody was raised against a peptide corresponding to the C-terminus of human Suz12.

Isotype: IgG

Application Details

Application Notes: ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB*: 1:250 - 1:1,000 dilution CUT&RUN: 1 µL per 50 µL reaction *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. An additional band at approximately 20 kDa is also detected by this antibody in HeLa and HEK lysates, but not in mouse CSML0 cell lysate. For Suz12, we also offer AbFlex Suz12 Recombinant Antibody (rAb). For details, see Catalog No. 91163.

Restrictions: For Research Use only

Handling

Buffer: PBS, 30 % glycerol, 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for SUZ12

Target: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Alternative Name: Suz12

Background:

Background_Plain: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.

Aliases: CHET9, JJAZ1

Molecular Weight: 95 kDa

NCBI Accession: NP_056170