Recombinant UBE2S antibody

Catalog No. ABIN7938534

The Rabbit Monoclonal anti-UBE2S antibody (Clone 6D18) (ABIN7938534) specifically detects UBE2S in WB and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for Recombinant UBE2S antibody (ABIN7938534)

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This UBE2S antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 6D18

Product Details anti-UBE2S Antibody

Purpose: UBE2S antibody (rAb), 100 μg (Clone 6D18)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against the UBE2S fusion protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:50000 dilution IHC: 1:2000-1:10000 dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, pH 7.3, with 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for UBE2S

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Alternative Name: UBE2S

Background:

Background_Plain: UBE2S (Ubiquitin-conjugating enzyme E2 S) also known as E2-EPF, E2EPF and EPF5. UBE2S is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2S can accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro, it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

Aliases: Ubiquitin conjugating enzyme E2 S, E2-EPF, E2EPF, EPF5

Molecular Weight: 24 kDa

Pathways: Ubiquitin Proteasome Pathway