YTHDF2 antibody (C-Term)

Catalog No. ABIN7938549

The Rabbit Polyclonal anti-YTHDF2 antibody is suitable to detect YTHDF2 in samples from Human. It has been validated for WB.

Quick Overview for YTHDF2 antibody (C-Term) (ABIN7938549)

Target: YTHDF2 (YTH Domain Family, Member 2 (YTHDF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YTHDF2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-YTHDF2 Antibody

Binding Specificity: C-Term

Purpose: YTHDF2 antibody (pAb)

Purification: Affinity Purified

Immunogen: This YTHDF2 antibody was raised against a fusion protein with His-tags at the C-terminus.

Isotype: IgG

Application Details

Application Notes: WB: 1 - 4 μg/mL

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, 30 % glycerol, 0.035 % Azide. Sodium Azide is highly toxic.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for YTHDF2

Target: YTHDF2 (YTH Domain Family, Member 2 (YTHDF2))

Alternative Name: YTHDF2

Background:

Background_Plain: YTHDF2 (YT521-B homology domain family 2, also known as CAHL, HGRG8, NY-REN-2) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF proteins contain a highly conserved single-stranded RNA-binding domain which can specifically recognize and bind m6A-containing RNAs. An important role of YTHDF2is in regulating mRNA stability. YTHDF2 recognizes many m6A-containing mRNAs that are not being actively translated and recruits them to cytoplasmic processing (P) bodies for degradation. YTHDF2 preserves 5'UTR methylation of stress- induced transcripts and promotes cap-independent mRNA translation by competitive binding and limiting the m6A 'eraser' FTO from demethylation. N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).

Aliases: YTH N6-Methyladenosine RNA Binding Protein 2, HGRG8

Molecular Weight: 70 kDa

NCBI Accession: NP_057342