Recombinant YTHDF2 antibody

Catalog No. ABIN7938551

The Rabbit Monoclonal anti-YTHDF2 antibody is suitable to detect YTHDF2 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for Recombinant YTHDF2 antibody (ABIN7938551)

Target: YTHDF2 (YTH Domain Family, Member 2 (YTHDF2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This YTHDF2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 3L6

Product Details anti-YTHDF2 Antibody

Purpose: YTHDF2 antibody (rAb), 100 μg (Clone 3L6)

Purification: Protein A Chromatography

Immunogen: This antibody was raised against the YTHDF2 fusion protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:50000 dilution

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS, pH 7.3, with 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for YTHDF2

Target: YTHDF2 (YTH Domain Family, Member 2 (YTHDF2))

Alternative Name: YTHDF2

Background:

Background_Plain: YTHDF2 (YT521-B homology domain family 2, also known as CAHL, HGRG8, NY-REN-2) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF proteins contain a highly conserved single-stranded RNA-binding domain which can specifically recognize and bind m6A-containing RNAs. An important role of YTHDF2 is in regulating mRNA stability. YTHDF2 recognizes many m6A-containing mRNAs that are not being actively translated and recruits them to cytoplasmic processing (P) bodies for degradation. YTHDF2 preserves 5'UTR methylation of stress- induced transcripts and promotes cap-independent mRNA translation by competitive binding and limiting the m6A 'eraser' FTO from demethylation.

Aliases: YTH N6-Methyladenosine RNA Binding Protein 2, HGRG8

Molecular Weight: 62 kDa