YWHAE antibody (AA 1-255)

Catalog No. ABIN7964690

The Rabbit Polyclonal anti-YWHAE antibody is suitable to detect YWHAE in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF, FACS and ICC.

Quick Overview for YWHAE antibody (AA 1-255) (ABIN7964690)

Target: YWHAE (14-3-3 epsilon (YWHAE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-YWHAE Antibody

Binding Specificity: AA 1-255

Purpose: Anti-YWHAE Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-YWHAE Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human YWHAE recombinant protein (Position: M1-Q255).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for YWHAE

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: YWHAE

Background:

Background: 14-3-3 protein epsilon is a protein that in humans is encoded by the YWHAE gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

Gene Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon

Molecular Weight: 28 kDa

Gene ID: 7531

UniProt: P62258

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase