14-3-3 eta antibody (C-Term)

Catalog No. ABIN7964723

The Rabbit Polyclonal anti-14-3-3 eta antibody is suitable to detect 14-3-3 eta in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for 14-3-3 eta antibody (C-Term) (ABIN7964723)

Target: 14-3-3 eta (YWHAH)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 eta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-14-3-3 eta Antibody

Binding Specificity: C-Term

Purpose: Anti-YWHAH Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-YWHAH Antibody. Tested in WB, IF, IHC, ICC, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human YWHAH.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunofluorescence, 5 μg/mL, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for 14-3-3 eta

Target: 14-3-3 eta (YWHAH)

Alternative Name: YWHAH

Background:

Background: 14-3-3 protein eta also referred to as 14-3-3η is a protein that in humans is encoded by the YWHAH gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.

Gene Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta

Molecular Weight: 28 kDa

Gene ID: 7533

UniProt: Q04917

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, C21-Steroid Hormone Metabolic Process, Myometrial Relaxation and Contraction