HAAO antibody (AA 52-286)

Catalog No. ABIN7964899

This Rabbit Polyclonal antibody specifically detects HAAO in WB, ELISA, IF and ICC. It exhibits reactivity toward Human and Rat.

Quick Overview for HAAO antibody (AA 52-286) (ABIN7964899)

Target: HAAO (3-hydroxyanthranilate 3,4-Dioxygenase (HAAO))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HAAO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-HAAO Antibody

Binding Specificity: AA 52-286

Purpose: Anti-HAAO Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human HAAO shares 86.3%,88.5% amino acid (aa) sequence identity with mouse,rat HAAO,respectively.

Characteristics: Anti-HAAO Antibody. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HAAO recombinant protein (Position: E52-G286). Human HAAO shares 86.3% and 88.5% amino acid (aa) sequence identity with mouse and rat HAAO, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HAAO

Target: HAAO (3-hydroxyanthranilate 3,4-Dioxygenase (HAAO))

Alternative Name: HAAO

Background:

Background: 3-hydroxyanthranilate 3,4-dioxygenase (EC 1.13.11.6) is an enzyme encoded by the HAAO gene. 3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN.

Gene Full Name: 3-hydroxyanthranilate 3,4-dioxygenase

Molecular Weight: 33 kDa

Gene ID: 23498

UniProt: P46952