HIBADH antibody (AA 34-332)

Catalog No. ABIN7964921

The Rabbit Polyclonal anti-HIBADH antibody is suitable to detect HIBADH in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for HIBADH antibody (AA 34-332) (ABIN7964921)

Target: HIBADH (3-hydroxyisobutyrate Dehydrogenase (HIBADH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIBADH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-HIBADH Antibody

Binding Specificity: AA 34-332

Purpose: Anti-HIBADH Antibody

Predicted Reactivity: Human HIBADH shares 93.3%,92.6% amino acid (aa) sequence identity with mouse,rat HIBADH,respectively.

Characteristics: Anti-HIBADH Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HIBADH recombinant protein (Position: R34-E332). Human HIBADH shares 93.3% and 92.6% amino acid (aa) sequence identity with mouse and rat HIBADH, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HIBADH

Target: HIBADH (3-hydroxyisobutyrate Dehydrogenase (HIBADH))

Alternative Name: HIBADH

Background:

Background: In enzymology, a 3-hydroxyisobutyrate dehydrogenase also known as β-hydroxyisobutyrate dehydrogenase or 3-hydroxyisobutyrate dehydrogenase, mitochondrial (HIBADH) is an enzyme that in humans is encoded by the HIBADH gene. This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde.

Gene Full Name: 3-hydroxyisobutyrate dehydrogenase

Molecular Weight: 32 kDa

Gene ID: 11112

UniProt: P31937