PFKFB4 antibody (AA 1-298)

Catalog No. ABIN7965252

The Rabbit Polyclonal anti-PFKFB4 antibody (ABIN7965252) specifically detects PFKFB4 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for PFKFB4 antibody (AA 1-298) (ABIN7965252)

Target: PFKFB4 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 4 (PFKFB4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKFB4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Carrier-free

Product Details anti-PFKFB4 Antibody

Binding Specificity: AA 1-298

Purpose: Anti-PFKFB4 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PFKFB4 shares 97.3%,97% amino acid (aa) sequence identity with mouse,rat PFKFB4,respectively.

Characteristics: Anti-PFKFB4 Antibody. Tested in WB, ELISA applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PFKFB4 recombinant protein (Position: M1-K298). Human PFKFB4 shares 97.3% and 97% amino acid (aa) sequence identity with mouse and rat PFKFB4, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PFKFB4

Target: PFKFB4 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 4 (PFKFB4))

Alternative Name: PFKFB4

Background:

Background: 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 4 also known as PFKFB4 is an enzyme which in humans is encoded by the PFKFB4 gene. The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2, 6-bisphosphate (F2, 6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2, 6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4

Molecular Weight: 54 kDa

Gene ID: 5210

UniProt: Q16877