Aconitase 1 antibody (AA 1-137) (HRP)

Catalog No. ABIN7965589

This Rabbit Polyclonal antibody specifically detects Aconitase 1 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for Aconitase 1 antibody (AA 1-137) (HRP) (ABIN7965589)

Target: Aconitase 1 (ACO1)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Aconitase 1 Antibody (HRP)

Binding Specificity: AA 1-137

Purpose: Anti-Aconitase 1/ACO1 Antibody HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Aconitase 1/ACO1 recombinant protein (Position: M1-D137).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: ACO1

Background:

Background: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants.

Gene Full Name: aconitase 1

Gene ID: 48

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis