beta Actin antibody (N-Term) (HRP)

Catalog No. ABIN7965699

The HRP-conjugated Rabbit Polyclonal anti-beta Actin antibody (ABIN7965699) specifically detects beta Actin in WB, IHC and ELISA. The antibody is reactive with Human, Mouse, Rat, Chicken, Monkey and Zebrafish (Danio rerio) samples.

Quick Overview for beta Actin antibody (N-Term) (HRP) (ABIN7965699)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-beta Actin Antibody (HRP)

Binding Specificity: N-Term

Purpose: Anti-beta Actin/ACTB Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB

Background:

Background: beta-Actin (ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al. (1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al. (2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al. (2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.

Gene Full Name: actin beta

Sequence Similarities: Belongs to the actin family.

Gene ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction