AFT1 antibody (AA 1-271) (HRP)

Catalog No. ABIN7965820

This Rabbit Polyclonal antibody specifically detects AFT1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for AFT1 antibody (AA 1-271) (HRP) (ABIN7965820)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFT1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-AFT1 Antibody (HRP)

Binding Specificity: AA 1-271

Purpose: Anti-ATF1 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human ATF1 shares 91% amino acid (aa) sequence identity with mouse ATF1.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATF1 recombinant protein (Position: M1-V271). Human ATF1 shares 91% amino acid (aa) sequence identity with mouse ATF1.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Alternative Name: ATF1

Background:

Background: ATF1, also known as activating transcription factor 1, is a protein that in humans is encoded by the ATF1 gene. It is mapped to 12q13.12. This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.

Gene Full Name: activating transcription factor 1

Sequence Similarities: Belongs to the bZIP family. ATF subfamily.

Gene ID: 466

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades