ZAP70 antibody (pTyr315, pTyr319) (Biotin)

Catalog No. ABIN746935

This Rabbit Polyclonal antibody specifically detects ZAP70 in WB, ELISA and IHC (fro). It exhibits reactivity toward Human and Mouse.

Quick Overview for ZAP70 antibody (pTyr315, pTyr319) (Biotin) (ABIN746935)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ZAP70 Antibody (Biotin)

Binding Specificity: pTyr315, pTyr319

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyr315/Tyr319

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70 +

Background:

Synonyms: Zap-70Tyr315/Tyr319, Zap-70Y315/Y319, p-ZAP70 Tyr315/Tyr319, p-ZAP-70 Tyr315/Tyr319, p-ZAP70 Y315/Y319, ZAP-70, ZAP 70, ZAP70, zeta-associated protein 70, ZAP-70=protein tyrosine kinase Syk homolog {SH2-like and C-terminal kinase domains}, Tyrosine-protein kinase ZAP-70, 70 kDa zeta-associated protein, Syk-related tyrosine kinase. SRK, STD, TZK, ZAP70_HUMAN, Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein.

Background: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Gene ID: 7535

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway