APRT antibody (C-Term)

Catalog No. ABIN7966605

The Rabbit Polyclonal anti-APRT antibody (ABIN7966605) specifically detects APRT in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for APRT antibody (C-Term) (ABIN7966605)

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APRT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-APRT Antibody

Binding Specificity: C-Term

Purpose: Anti-Aprt Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Aprt Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse APRT, which shares 86.4% and 95.5% amino acid (aa) sequence identity with human and rat APRT, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for APRT

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Alternative Name: Aprt

Background:

Background: Adenine phosphoribosyltransferase (APRTase) is anenzymeencoded by theAPRTgene, found inhumanson chromosome 16. It belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: adenine phosphoribosyl transferase

Molecular Weight: 20 kDa

Gene ID: 11821

UniProt: P08030

Pathways: Ribonucleoside Biosynthetic Process