ALDH1A3 antibody (AA 37-154)

Catalog No. ABIN7967430

The Rabbit Polyclonal anti-ALDH1A3 antibody (ABIN7967430) specifically detects ALDH1A3 in WB, IHC, ICC, FACS and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ALDH1A3 antibody (AA 37-154) (ABIN7967430)

Target: ALDH1A3 (Aldehyde Dehydrogenase 1 Family, Member A3 (ALDH1A3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDH1A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-ALDH1A3 Antibody

Binding Specificity: AA 37-154

Purpose: Anti-ALDH1A3 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human ALDH1A3 shares 89%,87.3% amino acid (aa) sequence identity with mouse,rat ALDH1A3,respectively.

Characteristics: Anti-ALDH1A3 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALDH1A3 recombinant protein (Position: N37-K154). Human ALDH1A3 shares 89% and 87.3% amino acid (aa) sequence identity with mouse and rat ALDH1A3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ALDH1A3

Target: ALDH1A3 (Aldehyde Dehydrogenase 1 Family, Member A3 (ALDH1A3))

Alternative Name: ALDH1A3

Background:

Background: Aldehyde dehydrogenase 1 family, member A3, also known as ALDH1A3 or retinaldehyde dehydrogenase 3 (RALDH3), is an enzyme that in humans is encoded by the ALDH1A3 gene. Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or a cellular retinol-binding protein form. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants.

Gene Full Name: aldehyde dehydrogenase 1 family member A3

Molecular Weight: 50-56 kDa

Gene ID: 220

UniProt: P47895

Pathways: Retinoic Acid Receptor Signaling Pathway