ALPL antibody (N-Term) (Biotin)

Catalog No. ABIN7967750

The Rabbit Polyclonal anti-ALPL antibody is suitable to detect ALPL in samples from Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for ALPL antibody (N-Term) (Biotin) (ABIN7967750)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALPL antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ALPL Antibody (Biotin)

Binding Specificity: N-Term

Purpose: Anti-Alkaline Phosphatase/ALPL Antibody Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of rat Alkaline Phosphatase, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: ALPL

Background:

Background: Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme that in humans is encoded by the ALPL gene. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Liver/bone/kidney alkaline phosphatase, also known as tissue-nonspecific alkaline phosphatase, acts physiologically as a lipid-anchored phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) ectophosphatase Mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked ly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.

Gene Full Name: alkaline phosphatase, biomineralization associated

Sequence Similarities: Contains 12 fibronectin type-I domains.

UniProt: P08289