ANG antibody (AA 25-147)

Catalog No. ABIN7968431

The Rabbit Polyclonal anti-ANG antibody is suitable to detect ANG in samples from Human. It has been validated for WB, IHC, ELISA, ICC, FACS and IF.

Quick Overview for ANG antibody (AA 25-147) (ABIN7968431)

Target: ANG (Angiogenin (ANG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-ANG Antibody

Binding Specificity: AA 25-147

Purpose: Anti-Angiogenin/ANG Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human Angiogenin,ANG recombinant protein (Position: Q25-P147). Human Angiogenin,ANG shares 77.5% amino acid (aa) sequence identity with mouse Angiogenin,ANG.

Characteristics: Anti-Angiogenin/ANG Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Angiogenin/ANG recombinant protein (Position: Q25-P147). Human Angiogenin/ANG shares 77.5% amino acid (aa) sequence identity with mouse Angiogenin/ANG.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human ELISA(Cap) , 1-5 μg/mL, Human, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ANG

Target: ANG (Angiogenin (ANG))

Alternative Name: ANG

Background:

Background: Angiogenin (Ang), also known as ribonuclease 5, is a small 123 amino acid protein that in humans is encoded by the ANG gene. The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region.

Gene Full Name: angiogenin

Molecular Weight: 17 kDa

Gene ID: 283

UniProt: P03950