Erythrocyte Ankyrin antibody (AA 1300-1844) (HRP)

Catalog No. ABIN7968790

This Mouse Monoclonal antibody specifically detects Erythrocyte Ankyrin in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Erythrocyte Ankyrin antibody (AA 1300-1844) (HRP) (ABIN7968790)

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Erythrocyte Ankyrin antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 5H2E8

Product Details anti-Erythrocyte Ankyrin Antibody (HRP)

Binding Specificity: AA 1300-1844

Purpose: Anti-Ankyrin erythroid/ANK/ANK1 Antibody (monoclonal, 5H2E8) HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Ankyrin erythroid/ANK/ANK1 recombinant protein (Position: N1300-Q1844).

Isotype: IgG2a

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Erythrocyte Ankyrin

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Alternative Name: ANK1

Background:

Background: Ankyrin 1, erythrocytic, also known as ANK1, is a protein that in humans is encoded by the ANK1 gene. Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats, a central region with a highly conserved spectrin binding domain, and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.

Gene Full Name: ankyrin 1

Gene ID: 286

UniProt: P16157

Pathways: Synaptic Membrane