APOA1 antibody (AA 25-259) (Cy3)

Catalog No. ABIN7969291

The Rabbit Polyclonal anti-APOA1 antibody is suitable to detect APOA1 in samples from Rat. It has been validated for FACS.

Quick Overview for APOA1 antibody (AA 25-259) (Cy3) (ABIN7969291)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOA1 antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-APOA1 Antibody (Cy3)

Binding Specificity: AA 25-259

Purpose: Anti-Apolipoprotein A I/APOA1 Antibody Cy3 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived rat APOA1 recombinant protein (Position: D25-A259). Rat APOA1 shares 61.7%,68.6% amino acid (aa) sequence identity with human,mouse APOA1,respectively.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat APOA1 recombinant protein (Position: D25-A259). Rat APOA1 shares 61.7% and 68.6% amino acid (aa) sequence identity with human and mouse APOA1, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: Apoa1

Background:

Background: Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.

Gene Full Name: apolipoprotein A1

Gene ID: 25081

UniProt: P04639

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism