AQP2 antibody (AA 102-271) (PE)

Catalog No. ABIN7969672

The Rabbit Polyclonal anti-AQP2 antibody is suitable to detect AQP2 in samples from Rat and Mouse. It has been validated for FACS.

Quick Overview for AQP2 antibody (AA 102-271) (PE) (ABIN7969672)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP2 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Product Details anti-AQP2 Antibody (PE)

Binding Specificity: AA 102-271

Purpose: Anti-Aqp2 Antibody PE Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Mouse Aqp2 shares 87.6%,98.2% amino acid (aa) sequence identity with human,rat Aqp2,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Aqp2 recombinant protein (Position: A102-A271). Mouse Aqp2 shares 87.6% and 98.2% amino acid (aa) sequence identity with human and rat Aqp2, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Alternative Name: Aqp2

Background:

Background: AQP2 (Aquaporin 2), also called AQUAPORIN-CD, is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. The investigators suggested that a defect in the AQP2 gene is the basis of the autosomal form of nephrogenic diabetes insipidus. The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aqp2, AQP2 trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide (NO). The NO donors sodium nitroprusside (SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin (VP), stimulating relocation of Aqp2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. AQP2 expression in kidney connecting tubules is sufficient for survival and that AQP2 expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the Aqp2 protein prevented phosphorylation at S256 and the subsequent accumulation of Aqp2 on the apical membrane of the collecting duct principal cells.

Gene Full Name: aquaporin 2

Gene ID: 11827

UniProt: P56402

Pathways: Response to Water Deprivation