ATG7 antibody (AA 7-690)

Catalog No. ABIN7970655

This Rabbit Polyclonal antibody specifically detects ATG7 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for ATG7 antibody (AA 7-690) (ABIN7970655)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-ATG7 Antibody

Binding Specificity: AA 7-690

Purpose: Anti-ATG7 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ATG7 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATG7 recombinant protein (Position: D7-Q690).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7

Background:

Background: Autophagy related 7 is a protein in humans encoded by ATG7 gene. It is mapped to 3p25.3. This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.

Gene Full Name: autophagy related 7

Molecular Weight: 78 kDa

Gene ID: 10533

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy